Fig. 6: Excessive GRP78 facilitates the membrane translocation of MRP1. | Cell Death & Disease

Fig. 6: Excessive GRP78 facilitates the membrane translocation of MRP1.

From: Tumor-associated macrophages confer colorectal cancer 5-fluorouracil resistance by promoting MRP1 membrane translocation via an intercellular CXCL17/CXCL22–CCR4–ATF6–GRP78 axis

Fig. 6

A QRT-PCR was applied to detect the expression of transports (LRP, BCRP, MRP-1, and MDR) in DLD1, SW480, and SW620 cells which were treated with 20% TAMs-CM-T. B QRT-PCR was applied to detect the expression of transports (LRP, BCRP, MRP-1, and MDR) in DLD1, SW480, and SW620 cells which were treated with 20% TAMs-CM-H. C Western blot was applied to detect the expression of transports in DLD1 and SW480 cells treated with 20% TAMs-CM-T. D After isolating the cytomembrane, the western blot was applied to detect the membrane translocation of the transports in DLD1 and SW480 cells treated with 20% TAMs-CM-T. E Knocking down GRP78, the membrane translocation of MRP1 was detected by western blot in DLD1 and SW480 cells which were treated with 20% TAMs-CM-T. F Knocking down GRP78, the membrane translocation of MRP1 was detected by immunofluorescence in DLD1 and SW480 cells which were treated with 20% TAMs-CM-T. G After knocking down GRP78 by shRNA, or inhibiting MRP1 by MK-571, 20% TAMs-CM-T were applied to evaluate its effects on the 5-FU efflux of DLD1 by HPLC. H The bar graph represented the concentration of 5-FU in DLD1 cells that were shown in G, **p < 0.01. I After inhibiting MRP1 by MK-571, the viability of cells was detected in DLD1 and SW480 cells which were treated with 20% TAMs-CM-H, **p < 0.01. J After knockdown MRP1 by siRNA, the viability of cells was detected in DLD1 and SW480 cells which were treated with 20% TAMs-CM-H, **p < 0.01.

Back to article page