Fig. 7: GRP78 interacts and co-translocates with MRP1 to cytomembrane. | Cell Death & Disease

Fig. 7: GRP78 interacts and co-translocates with MRP1 to cytomembrane.

From: Tumor-associated macrophages confer colorectal cancer 5-fluorouracil resistance by promoting MRP1 membrane translocation via an intercellular CXCL17/CXCL22–CCR4–ATF6–GRP78 axis

Fig. 7

A After isolating the cytomembrane, the membrane translocation of GRP78 was detected by western blot in DLD1 and SW480 cells treated with 20% TAMs-CM-T. B The detachment of the KDEL receptor (KDELR) from Golgi was detected by immunofluorescence stain, KDELR (green), GM130 marked Golgi (red). C The interaction of GRP78 and MRP1 was detected by Co-IP in DLD1 cells. D The interaction of GRP78 and MRP1 was detected by Co-IP in GRP78-overexpressed DLD1 cells. E With 20% TAMs-CM-T treatment, the colocation of GRP78 and MRP1 was detected by immunofluorescence stain, GRP78 (green), MRP1 (red). F With 20% TAMs-CM-T and 20% TAMs-CM-H treatment, the expression of GRP78 was detected by western blot according to the gradient of time. G With 20% TAMs-CM-T treatment, the expression of GRP78 and MRP1 in ER, cytoplasm, and cytomembrane were detected according to the gradient of time. H GRP78 (T500), GRP78 (T280), and GRP78 (C154) were overexpressed in DLD1 cells, and the Co-IP was applied to detect the interaction of MRP and deletion mutants of GRP78. I GRP78 (WT) and GRP78 (T453D) were overexpressed in DLD1, and the Co-IP was applied to detect the interaction of MRP and GRP78 (T453D). J After overexpressing GRP78 (WT) and GRP78 (T453D), the 5-FU in DLD1 was detected by HPLC, **p < 0.01. K After overexpressing GRP78 (WT) and GRP78 (T453D), the viability of the cell was detected in DLD1 and SW480, which were treated with 40 μM 5-FU, **p < 0.01. L After overexpressing GRP78 (WT) and GRP78 (T453D), the formation of the cell colony was detected in DLD1 cells, which were treated with 40 μM 5-FU, **p < 0.01.

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