Fig. 1: BAPTAi induced apoptosis in multiple hematological lines. | Cell Death & Disease

Fig. 1: BAPTAi induced apoptosis in multiple hematological lines.

From: Intracellular BAPTA directly inhibits PFKFB3, thereby impeding mTORC1-driven Mcl-1 translation and killing MCL-1-addicted cancer cells

Fig. 1

Quantitative analysis of apoptosis of OCI-LY-1 (A, N = 3), SU-DHL-4 (B, N = 6), H929 (C, N = 3), OCI-AML-2 (D, N = 3), OCI-AML-3 (E, N = 3) and WEHI-231 (F, N = 3) cells at different time points after the addition of vehicle (DMSO, blue) or 10 µM BAPTA-AM (red). Cells were stained with annexin V-FITC and 7-AAD, and the apoptotic fraction was identified as annexin V-positive cells. Data are represented as the average ± S.D. (N = 3). Statistical significance of differences was determined with a paired, two-tailed t-test. Differences were considered significant when p < 0.05 (**p < 0.01; ***p < 0.001).

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