Fig. 5: Ketogenic diet suppresses PanNET progression.

A Islet areas of 30-week-old wild-type mice and Men1^f/f-RipCre⁺ mice fed a normal diet were analyzed. The areas of the top 10 largest islets were calculated, and the means of the 10 islet areas were obtained for each mouse. Data are represented as the mean ± SD. B Pancreatic sections of 45-week-old Men1^f/f-RipCre⁺ mice fed a normal diet or ketogenic diet from 30 weeks of age were stained with HE. HE staining of pancreatic sections of 30-week-old Men1^f/f-RipCre⁺ mice fed a normal diet is also shown. C Islet areas of 45-week-old Men1^f/f-RipCre⁺ mice fed a normal diet or ketogenic diet from 30 weeks of age and 10- or 30-week-old Men1^f/f-RipCre⁺ mice fed a normal diet were analyzed as in (A). Men1^f/f-RipCre⁺ mice fed a normal diet are the same as the mice described in (A). Data are represented as the mean ± SD. D Statistical analysis of the average islet sizes of each group of mice was performed. P values were obtained using the one-way ANOVA test. The ages of the mice sacrificed are shown in parentheses. E, F Pancreatic sections of 45-week-old Men1^f/f-RipCre⁺ mice fed a normal diet (N = 3) or ketogenic diet from 30 weeks of age (N = 3) were stained with anti-Ki67 (E). Graph F shows the number of total versus Ki67-positive nuclei from 4 islets. Data are represented as the mean ± SD. G, H HE-stained pancreatic sections of 45-week-old Men1^f/f-RipCre⁺ mice fed a normal diet (N = 4) or Men1^f/f-RipCre⁺ mice fed a ketogenic diet from 30 weeks of age (N = 4) were analyzed as shown in Fig. 4B. The numbers of vessels are shown in graph H. In F, H, data are represented as the mean ± SD.