Fig. 2: CXCR1 modulates the production of inflammatory cytokines in DCs.

A DCs sorted from WT and Cxcr1^−/− mice and treated with LPS (100 ng/ml) for 24 h. IL-6 and IL-12p70 in DCs supernatant were examined by ELISA. B Representative flow cytometry data showing intracellular production of IFN-γ and IL-17A in CD4⁺ T cells from DC-T cell coculture system in vitro. C Pooled data are presented from (B). D ELISA detection of IFN-γ and IL-17A in supernatants of cocultured DC-T cells. E DCs from WT or Cxcr1^−/− mice were stimulated with LPS for 24 h and cultured in a transwell to naïve CD4⁺ T cell differentiation. F Representative flow cytometry data showing intracellular production of IFN-γ and IL-17A in CD4⁺ T cells. G, H The percentage of positive cells and protein levels of IL-17A (G) and IFN-γ (H) in cocultured DC-T cells or their supplemented. Pooled data are presented in the right panel. I Schematic diagram of cell adoptive transfer. J Representative flow cytometry data showing intracellular production of IFN-γ and IL-17A in CD4⁺ T cells 7 days after transfer. Pooled data are presented in the right panel. Data are mean ± SEM (n = 5–6). ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 vs. WT or indicated group. (one-tailed Student’s t-test). Data are representative of three independent experiments with similar results.