Fig. 2: Neat1-silenced mice developed alleviated EAU and displayed decreased Th17 cell responses. | Cell Death & Disease

Fig. 2: Neat1-silenced mice developed alleviated EAU and displayed decreased Th17 cell responses.

From: LncRNA Neat1 targets NonO and miR-128-3p to promote antigen-specific Th17 cell responses and autoimmune inflammation

Fig. 2

A Schematic diagram of the experimental procedure to study the role of Neat1 in EAU. B Real-time qRT-PCR analysis of Neat1 expression in eyes, spleens, and lymph nodes of mice infected with LV-Ctrl or LV-shNeat1 (n = 6 per group). C Clinical score of EAU in mice infected with LV-Ctrl and LV-shNeat1 (n = 6 per group). D Representative images of eyes in LV-Ctrl and LV-shNeat1-infected EAU mice on day 21 after EAU induction by retinal imaging microscope. E Representative images showing fundus condition by SD-OCT. Hyper-reflective foci (blue asterisk) in the vitreous cavity and retinal folds (yellow arrows) were observed around the optical nerve. F Histopathological analysis of eyes from lentivirus-infected EAU mice (n = 6 per group). Scale bars, 100 μm. G, H Flow cytometric analysis of the percentages of Th17 (G) and Th1 (H) in T cells from lentivirus-infected EAU mice (n = 6 per group). I Real-time qRT-PCR analysis of pathogenic Th17-related genes in T cells from lentivirus-infected EAU mice (n = 4 per group). Data are presented as mean ± SD of at least three independent experiments. *p < 0.05, **p < 0.01. See also Fig. S1.

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