Fig. 3: Neat1 downregulated in Th17 cells inhibits antigen-specific Th17 cell responses in vitro. | Cell Death & Disease

Fig. 3: Neat1 downregulated in Th17 cells inhibits antigen-specific Th17 cell responses in vitro.

From: LncRNA Neat1 targets NonO and miR-128-3p to promote antigen-specific Th17 cell responses and autoimmune inflammation

Fig. 3

A, C, D CD4+ T cells isolated from immunized mice were transfected with ASO-NC or ASO-Neat1 and stimulated with IRBP1-20 in the presence of irradiated APCs under Th17-polarizing conditions. A Real-time qRT-PCR analysis of Neat1 expression (n = 4 per group). C Flow cytometric analysis of the percentages of Th17 cells (n = 4 per group). D Real-time qRT-PCR analysis of Th17-related gene expression (n = 4 per group). B ELISA analysis of IL-17 production in CD4+ T cells transfected with ASO-NC or ASO-Neat1 and restimulated with the indicated antigens and IL-23 (n = 4 per group). Data are presented as mean ± SD of at least three independent experiments. *p < 0.05, **p < 0.01. See also Fig. S2.

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