Fig. 3: A151 alleviates cGAS–STING pathway activity, pyroptosis-associated proteins in vitro. | Cell Death & Disease

Fig. 3: A151 alleviates cGAS–STING pathway activity, pyroptosis-associated proteins in vitro.

From: Inhibition of cGAS–STING pathway alleviates neuroinflammation-induced retinal ganglion cell death after ischemia/reperfusion injury

Fig. 3

A The mRNA levels of cGAS or STING were determined using qRT–PCR (n = 3). B, C Western blot and quantitative analyses of cGAS, STING and phosphorylation of TBK1, p65 and IRF3 in BV2 cells (n = 3). D, E Western blot and quantitative analyses of GSDMD, Caspase-1 and IL-1β in BV2 cells (n = 3). F, G Immunofluorescence was conducted to detect Iba-1, iNOS and CD206 expression levels in BV2 cells. Scale bar: 20 μm. H Quantitative analysis of iNOS and CD206 (n = 3). I, J The mRNA levels of pro-inflammatory and anti-inflammatory cytokines, including IL-6, TNF-α, IL-1β, IFN-β, IL-10 and Arg-1 (n = 3) Data are shown as means ± SEM. The dots represent biological replicates. #P < 0.05, ##P < 0.01, ###P < 0.001 compared with the untreated group. *P < 0.05, **P < 0.01, ***P < 0.001 compared with the LPS + poly(dA:dT) group. One-way ANOVA followed by a Bonferroni post hoc test.

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