Fig. 4: IGF2BP2 modulates CSF2 m6A modification to induce MSC reprogramming.
From: IGF2BP2-meidated m6A modification of CSF2 reprograms MSC to promote gastric cancer progression
A In vitro tropism of MSCs towards HGC-27 cells assessed via a transwell system. Original magnification: ×100. Scale bar = 50 μm. B, C qRT-PCR (B) and western blot (C) analyses of FAP and α-SMA expression in MSCs from different groups. D Luminex analyses of the inflammatory factor profile in the supernatants from MSCs. E, F The proliferation of HGC-27 cells following exposure to the supernatants from different MSCs was assessed by cell viability (E) and colony formation assays (F). G Transwell assay for the migration of HGC-27 cells upon treatment with the supernatants from different MSCs. Original magnification: ×100. Scale bar = 50 μm. H CCK-8 assay for IC50 of 5-FU in HGC-27 cells treated with the supernatants from different MSCs. I Flow cytometric analyses of the apoptotic rate of HGC-27 cells after exposure to 5-FU. *P < 0.05; **P < 0.01; ***P < 0.001.
