Fig. 5: IL-33/ST2 upregulated SLC7A11 by regulating ATF3.

A Venn graph showed the intersection of the differential expression gene in Macrophage co-culture treated eESCs and Ferroptosis marker (data originated from GSE 19834 and FerroDb). B Representative immunohistochemical images staining with ATF3 in normal endometrial tissue (EN) (n = 8) and ectopic endometriosis lesion tissue (EC) (n = 8) (original magnification ×200). C Western blot was used to detect the protein levels of ATF3 in nESCs and eESCs. D Western blot was used to detect the protein levels of ATF3 in eESCs treated with or without rIL-33 (100 ng/ml). E Western blot was used to determine the knockdown efficiency of si-ATF3 in eESCs. F Western blot was used to detect the protein levels of SLC7A11 and GPX4 in different groups: sicontrol (50 nM), siATF3 (50 nM), siST2 (50 nM) and double transfection of siATF3 (50 nM) and siST2 (50 nM). G The ChIP-seq data previously reported were reanalyzed.(GSM1917770, ENCSR632DCH_2, GSM803508, GSM803503). H Chromatin immunoprecipitation assay (CHIP) was used to verify the binding region of ATF3 and SLC7A11 promoter. Data are presented as the mean ± SD, n = 3 independent experiments. Statistical analysis was performed using Student’s t test. ****p < 0.0001, **p < 0.01, *p < 0.05. ATF3 activating transcription factor, siATF3 siRNA targeting ATF3, siAT+siST siRNA targeting ATF and siRNA targeting ST2.