Fig. 6: Loss of Kindlin-2 activates STAT3 and upregulates Dll1 in myoepithelium.
From: Kindlin-2 in myoepithelium controls luminal progenitor commitment to alveoli in mouse mammary gland
A Multi-color staining showing CK5 (green), STAT3 (orange), STAT3 pY705 (cyan), Dll1 (red), and DAPI (blue) of P18.5 mammary gland in K14-Cre + ; Kindlin-2flox/flox or K14-Cre-; Kindlin-2flox/flox females. B Statistical analysis showing the proportion of STAT3+ cells in CK5+ cells, STAT3 pY705+ cells in CK5+ cells, and Dll1+ cells in CK5+ cells at P18.5 in K14-Cre + ; Kindlin-2flox/flox group compared with K14-Cre-; Kindlin-2flox/flox group (n = 5 fields of view at random per genotype). Statistical testing was performed by unpaired t test. Data are presented as mean values ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. C Multi-color staining showing CK5 (green), STAT3 (orange), STAT3 pS727 (cyan), Dll1 (red), and DAPI (blue) of P18.5 mammary gland in K14-Cre + ; Kindlin-2flox/flox or K14-Cre-; Kindlin-2flox/flox females. D Statistical analysis showing the proportion of STAT3+ cells in CK5+ cells, STAT3 pS727+ cells in CK5+ cells, and Dll1+ cells in CK5+ cells at P18.5 in K14-Cre + ; Kindlin-2flox/flox group compared with K14-Cre-; Kindlin-2flox/flox group (n = 5 fields of view at random per genotype). Statistical testing was performed by unpaired t test. Data are presented as mean values ± SD. *P < 0.05, **P < 0.01. E Scatter plot showing the distribution and Pearson’s correlation of Dll1 and STAT3 pY705/pS727 fluorescence intensity in CK5+ cells from multiple fluorescence strain.
