Fig. 6: LDHA is essential for FOXQ1-mediated PC cell proliferation, stemness, and aerobic glycolysis in vitro.

Small interfering RNA of LDHA (si-LDHA) and 2-DG (glycolytic pathway inhibitors) were added to PANC-1 and CFPAC-1 cells infected with lentivirus that overexpressed FOXQ1. A CCK-8 evaluation of cell proliferation of each group. B Sphere formation experiment. C Colony formation assay of each group. D EdU staining images of the indicated cell groups. E ECAR assay of the indicated cell groups. F OCR assay of the indicated cell groups. Scale bar: 100 µm; *p < 0.05; **p < 0.01.