Fig. 5: SYVN1 enhances glycolysis and lactate export in LUAD cells.

A–D Lactate production and glucose consumption were monitored at the indicated time points in A549 and H1752 cells infected with lentiviruses carrying scramble shRNA (scramble) or shRNAs targeting SYVN1. Data are presented as the mean ± SD (**P < 0.01, n = 5, two-way ANOVA). E, F LDH activity is compromised in A549 (E) and H1752 (F) cells upon SYVN1 knockdown. Data are presented as the mean ± SD (*P < 0.05, **P < 0.01, n = 3, two-tailed unpaired t test). G–J SYVN1 knockdown alters the extracellular acidification rate (ECAR). A549 and H1752 cells were infected with lentiviruses carrying the indicated shRNA. The ECAR was assessed after glucose was added, oligomycin (oligo), and 2-deoxyglucose (2DG). G, I Time course of a representative experiment. H, J Determination of the glycolysis rate and glycolytic capacity. Data are presented as the mean ± SD (*P < 0.05, **P < 0.01, n = 3, two-tailed unpaired t test). K–N SYVN1 knockdown alters the oxygen consumption rate (OCR). A549 and H1752 cells were infected with lentiviruses carrying the indicated shRNA. OCR was measured after oligomycin, FCCP, and rotenone were added. K, M time course of a representative experiment. L, N determination of the OCR used for basal respiration and maximal respiration. Data are presented as the mean ± SD (*P < 0.05, **P < 0.01, n = 3, two-tailed unpaired t test). O, R Overexpression of MCT4 reverses lactate production (O, P) and glucose consumption (Q, R) in A549 and H1752 cells infected with scramble or shRNAs targeting SYVN1. OE indicates overexpression. Data are presented as the mean ± SD (*P < 0.05, **P < 0.01, n = 5, two-tailed unpaired t tests).