Fig. 6: SYVN1 promotes LUAD progression through metabolic reprogramming.

A–C A tumour formation assay was performed by injecting A549 cells infected with lentiviruses carrying scramble control shRNA (scramble) or shRNAs targeting SYVN1. Tumour volumes (A) and weights (B) were measured. Data are presented as the mean ± SD (**P < 0.01, n = 10, two-tailed unpaired t test for tumour weight analysis and two-way ANOVA for tumour volume analysis). D, E Representative images of IHC of the cell proliferation marker Ki67 and capillary marker CD31 in tumour sections from the xenograft models at the endpoint. The histogram shows the quantification of Ki67-positive cells and CD31+ capillary area density. Data are presented as the mean ± SD (*P < 0.05, **P < 0.01, n = 10 mice, two-tailed unpaired t test). Scale bar: Ki67, 100 μm; CD31, 250 μm. F, G Overexpression of MCT4 partially reverses the SYVN1 knockdown-induced inhibition of cell proliferation. Colony formation of SYVN1 scramble and SYVN1 knockdown with overexpression of MCT4 in A549 cells. (G) Data are quantified and presented as the mean ± SD (*P < 0.05, **P < 0.01, n = 5, two-tailed unpaired t test). H CCK8 assays of SYVN1 scramble and SYVN1 knockdown with overexpression of MCT4 in A549 cells. Data are presented as the mean ± SD (*P < 0.05, **P < 0.01, n = 5, two-way ANOVA). I, J EdU incorporation assays of SYVN1 scramble and SYVN1 knockdown with overexpression of MCT4 in A549 cells. J Data are quantified and presented as the mean ± SD (*P < 0.05, **P < 0.01, n = 6, two-tailed unpaired t test). Scale bar: 50 μm. K VIP values of different metabolites between A549 cells infected with lentiviruses carrying scramble control shRNA (scramble) or shRNAs targeting SYVN1 were detected by NMR analysis. L Lactate export in tumour tissues from A549 cells infected with lentiviruses carrying scramble control shRNA (scramble) or shRNAs targeting SYVN1 was detected by NMR analysis. Data are presented as the mean ± SD (**P < 0.01, n = 10 mice, two-tailed unpaired t test).