Fig. 7: Trip12 deletion potentiates TGFβ response in primary mouse intestinal organoids.
From: Control of TGFβ signalling by ubiquitination independent function of E3 ubiquitin ligase TRIP12
A Bright field images of mouse intestinal organoids treated with 1 ng/ml TGFβ for indicated time intervals. B Quantification of apoptotic looking regressing organoids from experiment in (A). C Immunofluorescence images of mouse intestinal organoids treated with 1 ng/ml TGFβ for 48 h. Proliferating ISCs and TA are labelled with EdU and stained using Click-iTΤΜ Alexa Fluor kit. Arrows point at regressing budding structures that lose EdU incorporation compared to controls. Nuclei are counterstained with DAPI. D Quantification of EdU positive cells in organoids from (C). EdU positive cells were quantified from three independent images taken at different levels on at least 3–5 different organoids from two independent experiments. E Immunofluorescence for CC3 positive apoptotic cells in organoids from indicated genotypes. Nuclei are counterstained with DAPI. F CC3 positive cells are quantified from three independent images taken at different levels on at least 3–5 different organoids from two independent experiments and presented as % of DAPI positive cells. Statistics were done by One-way ANOVA. Each dot in the plots represents a mean value of cell count from up to 3 independent images from two different experiments, p = * < 0.05 and ** < 0.01.
