Fig. 2: RhoA regulates microglial metabolic reprogramming during inflammation.
From: RhoA balances microglial reactivity and survival during neuroinflammation
HMC3 microglia expressing the ATP biosensor (A), Glucose biosensor (B), Lactate biosensor (C), or Pyruvate biosensor (D) were transfected with RhoA Q63L (red) or RhoA WT (blue) and exposed to LPS (1 µg/ml; 20 min) (n = 15-30 cells per group from 3 independent experiments for each biosensor). Primary cortical microglia expressing the ATP biosensor (E) or Lactate biosensor (F) were transfected with the RhoA Q63L (red) or RhoA WT (blue) and exposed to LPS (1 µg/ml; 20 min) (n = 6 cells per group from 3 independent experiments for each biosensor). Panels are time-lapse ratio images coded according to the pseudocolor ramps. Graphs (means and SD) display F490/F435 (A and E), FRET/Donor (B), and Donor/FRET (C, D, and F) ratio change at 0 (CT) and 20 min. G Seahorse measurements of bioenergetic parameters in HCM3 microglia expressing RhoA Q63L or RhoA WT. The parameters were calculated based on the OCR following the sequential addition of LPS, oligomycin, FCCP, rotenone, and antimycin A. Results are from at least 3 independent experiments. Graphs show the mean with SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Two-way ANOVA). Scale bars: 20 µm.
