Fig. 3: RhoA regulates microglial proinflammatory reactivity.
From: RhoA balances microglial reactivity and survival during neuroinflammation
HMC3 microglia expressing a ROS biosensor (A), AMPK biosensor (B), ERK biosensor (C), Src biosensor (D), and GFP-tagged p65 NFkB subunit (E) were transfected with the RhoA Q63L construct or with RhoA WT and then exposed to LPS (1 µg/ml for 20 min) (n = 18–100 cells per group from 3 independent experiments for each biosensor). Panels show time-lapse ratio images or mean fluorescent intensity (MFI) coded according to the pseudocolor ramps. F ELISA (TNF-α or IL-1ß) from culture supernatants of primary cortical microglia transfected with RhoA Q63L or RhoA WT and exposed to LPS (1 µg/ml) for 3 h (n = 4 independent experiments). Graphs are means with SD. *p < 0.05, ***p < 0.001, ****p < 0.0001 (Two-way ANOVA). Scale bars: 20 µm.
