Fig. 2: Overexpression of DNAJA4 suppresses NPC cell migration, invasion and EMT. | Cell Death & Disease

Fig. 2: Overexpression of DNAJA4 suppresses NPC cell migration, invasion and EMT.

From: DNAJA4 suppresses epithelial-mesenchymal transition and metastasis in nasopharyngeal carcinoma via PSMD2-mediated MYH9 degradation

Fig. 2

A In GSEA of public RNA-seq data (GSE102349), the gene sets related to metastasis and epithelial-mesenchymal transition (EMT) pathways exhibited high enrichment in NPC samples with low DNAJA4 expression. B Wound healing assay of HONE1 and SUNE1 cells transfected with the DNAJA4 overexpression or empty plasmid. C Transwell migration and invasion assays of HONE1 and SUNE1 cells transfected with the DNAJA4 overexpression or empty plasmid. D Representative western blot analysis of the epithelial marker E-cadherin and mesenchymal markers Vimentin, Slug, and Snail in HONE1 and SUNE1 cells transfected with the DNAJA4 overexpression or empty plasmid. E Representative immunofluorescence images of the epithelial marker E-cadherin and mesenchymal marker Vimentin in HONE1 cells transfected with the DNAJA4 overexpression or empty plasmids. Scale bar, 50 μm. The data in (B, C, E) are shown as mean ± SD, and p-values were determined by Student’s t test (*p < 0.05).

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