Fig. 2: AZD5991 induced apoptosis by displacing BAK and NOXA from MCL-1/BAK complex or MCL-1/NOXA complex.

A Whole cell extract (bottom) and co-IP of MCL-1 (top) was conducted using lysates from JeKo-1, JeKo-IBN-R, or JeKo BTK KD_2 cells after a 15-min treatment with AZD5991. B Time-dependent co-IP assay of MCL-1 with AZD5991 treatment in JeKo BTK KD_2 cells. C JeKo-1 cells were electroporated with BAK-targeted siRNAs followed by a subsequent 24-h exposure to AZD5991.