Fig. 7: Effects of ChemR23 activation on pyroptosis in SH-SY5Y cells after hypoglycaemic and hypoxic stimulation. | Cell Death & Disease

Fig. 7: Effects of ChemR23 activation on pyroptosis in SH-SY5Y cells after hypoglycaemic and hypoxic stimulation.

From: ChemR23 activation attenuates cognitive impairment in chronic cerebral hypoperfusion by inhibiting NLRP3 inflammasome-induced neuronal pyroptosis

Fig. 7

A Changes in cell viability with time under hypoglycaemic and hypoxic conditions. n = 4 per group. B, C Effects of different concentrations of RvE1 and C-9 on cell viability. n = 4 per group. D, E The proportion of Annexin V/PI double positive cells analysed by flow cytometry. n = 3 per group. F LDH levels in the cell culture supernatant. n = 3 per group. G–K Western blot analysis of NLRP3, GSDMD-N, Caspase-1 p20 and ASC. n = 3 per group. L, M Concentrations of IL-1β and IL-18 in the cell culture supernatant were measured by ELISA. n = 4 per group. (N) Representative immunofluorescence staining of NLRP3 (green) with the nuclei counterstained by DAPI. Scale bar: 50 μm. n = 3 per group. Data are presented as the mean ± SEM. **P < 0.01, ***P < 0.001 vs. Con; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. HH; &P < 0.05, &&P < 0.01, &&&P < 0.001 vs. HH+RvE1; †P < 0.05, ††P < 0.01, †††P < 0.001 vs. HH + C-9.

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