Fig. 8: ChemR23 activation inhibited neuronal pyroptosis via the PI3K/AKT/Nrf2 signalling pathway in SH-SY5Y cells. | Cell Death & Disease

Fig. 8: ChemR23 activation inhibited neuronal pyroptosis via the PI3K/AKT/Nrf2 signalling pathway in SH-SY5Y cells.

From: ChemR23 activation attenuates cognitive impairment in chronic cerebral hypoperfusion by inhibiting NLRP3 inflammasome-induced neuronal pyroptosis

Fig. 8

A Scanning electron microscopy analysis showing the cell morphology in different treatment groups. n = 3 per group. B Representative immunofluorescence staining of NLRP3 (green) with the nuclei counterstained by DAPI. Scale bar: 50 μm. n = 3 per group. C LDH levels in the cell culture supernatant. n = 3 per group. D, E ELISA of IL-1β and IL-18 levels. n = 3 per group. F–J The levels of NLRP3, GSDMD-N, Caspase-1 p20 and ASC were evaluated by western blot. n = 3 per group. K–N The expression levels of p-PI3K/PI3K, p-AKT/AKT and Nrf2 were evaluated by western blot. n = 3 per group. Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 vs. Con; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. HH; &P < 0.05, &&P < 0.01, &&&P < 0.001 vs. HH + OE; N.S. means not significant vs. HH + OE.

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