Fig. 2: AFs show a pro-inflammatory and chemotactic secretion profile.
From: Crosstalk with lung fibroblasts shapes the growth and therapeutic response of mesothelioma cells
A Cytokine,- chemokine and acute phase proteins profiling was performed on AFs obtained from incubation of MRC-5 fibroblasts with the CMs of the indicated MPM cell lines or Met-5A cells. Heatmap represent fold changes over treatment with Met-5A CM and K-mean clustering was performed on both proteome and CM conditions using ClusterMaker under Cytoscape. B Lysates from AFs and MRC-5 cells incubated with CM from Met-5A cells (NFMet) were analysed by SDS-PAGE/western blotting for the indicated proteins. Detection of βActin was used as a loading control. Left panel: representative blots. Right panel: Optical densitometry quantification of 4 biological replicates. Each dot represents an individual AF cell line. Statistics: Student t-test (*P < 0.05, **P < 0.01, ***P < 0.005). C Proteome profiling results from (A) were analysed by DAVID (https://david.ncifcrf.gov/) for functional interaction network and gene ontology. D Heat map of growth factor profiling assays performed on AFs obtained from incubation of NFs with MPM cell lines or Met-5A cells (NFMet). E Growth factors profiling results from (D) were analysed by DAVID (https://david.ncifcrf.gov/) for functional interaction network and gene ontology.
