Fig. 5: MPM/AF crosstalk results in the activation of a number of conserved signalling pathways.
From: Crosstalk with lung fibroblasts shapes the growth and therapeutic response of mesothelioma cells
Lysates from MRC-5 cells incubated for 3 days with CM from MPM cells (A) or from MPM cells treated for 3 days with CM of the corresponding AFs (B) were analysed by RPPA. Log2 fold changes in protein expression or post-translational modification over NFMet (A) or MPM cells treated with CM from NFMet (B) were averaged over all cell lines and data imported into Cytoscape to build a functional interaction network using the Reactome FI plugin. Lysates from the indicated AFs or NFMet (C) or from MPM or Met-5A cells treated or not for 3 days with CM of the corresponding AFs or NFMet, respectively (D) were analysed by SDS-PAGE/western blotting for the expression of the indicated proteins. Data representative of 3 independent biological repeats. E Three publicly-available microarray datasets were analysed for changes in the mRNA expression of the indicated targets between normal mesothelial and mesothelioma tissue samples (see corresponding Supplementary Fig. 9). Results were summarised as a heatmap with red indicating statistically significant increase, blue statistically significant decrease and grey no change in expression. These results are here compared with those of our RPPA and Western blotting data (same colour code). F The expression of SRC, PDGFRA, PDGFRB and MTOR was extracted from the TCGA Mesothelioma GDC RNA-Seq dataset and correlated with the proportion of fibroblast infiltration deduced by cellular deconvolution of bulk transcriptional data using the Kassandra algorithm. The p-values obtained from linear regression models and the Spearman correlation coefficients are shown.
