Fig. 2: GRSF1 modulates fibrinogen mRNA stability.

a–d GRSF1 overexpression reduces FGA, FGB, and FGG mRNAs half-life. 293T cells were transfected with pcDNA3.1 vector or pcDNA3.1-Flag-GRSF1 plasmid for 48 h and then exposed to Actinomycin D (2 μg/mL), and total RNAs were isolated at indicated times and subjected to real-time PCR to assess the half-life of FGA (b), FGB (c), and FGG (d) mRNAs. The half-life: GRSF1 OE vs Ctrl: (~4 h for FGA and FGB, ~3 h for FGG) vs (~5–6 h for FGA and FGB, ~4–5 h for FGG). e–h GRSF1 knockdown increases FGA, FGB, and FGG mRNAs half-life. 293T cells were transfected with siNC or siGRSF1, and then the half-life of FGA (f), FGB (g), and FGG (h) mRNAs were assessed by real-time PCR. The half-life: siGRSF1 vs siNC: (~7 h for FGA, FGB and FGG) vs (~5 h for FGA, FGB and FGG). Error bars in (b–d), and (f–h) represent as means ± SD from three independent experiments.