Fig. 5: Morphological changes and quantification of neointimal lesions in vein allografts. | Cell Death & Disease

Fig. 5: Morphological changes and quantification of neointimal lesions in vein allografts.

From: 3,4-dimethoxychalcone induces autophagy and reduces neointimal hyperplasia and aortic lesions in mouse models of atherosclerosis

Fig. 5

(A) A schematic overview of the experimental protocol is shown. (B) Representative images depict sections of vein segments from mice before grafting, or 4 weeks after isografting them into the carotid artery of mice and local treatment with vehicle (C) or 3,4-dimetoxychalcone (3,4-DC) (D). Tissue sections were stained with hematoxylin and eosin (B–D). Arrows indicate the endothelium in representative images. The luminal and neointimal areas were quantified by morphometry (E, F). The sections from vehicle-only treated (G) or 3,4-DC treated (H) mice were subjected to immunofluorescence staining with antibodies specific for the smooth muscle cell antigen α−actin-FITC (green) and counterstained with DAPI (blue; nuclei). Positive cells in the neointimal lesions were counted (I). Quantitative data indicate values for individual mice (n = 7). Data are represented as mean values ± SEM and significance was tested by Student’s t test.

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