Fig. 1: Brn-3b expression in aortic protein extracts and VSMC cultures. | Cell Death & Disease

Fig. 1: Brn-3b expression in aortic protein extracts and VSMC cultures.

From: Vascular dysfunction caused by loss of Brn-3b/POU4F2 transcription factor in aortic vascular smooth muscle cells is linked to deregulation of calcium signalling pathways

Fig. 1

a WB showing expression of the shorter Brn-3b(l) protein in aortic extracts prepared from pooled WT aortas (dissected from 2–3 mice) compared with pooled aortas from Brn-3b KO mutants (negative control). β−tubulin blots indicate variation in protein loading. b Representative images of immunostained WT aortic sections showing co-localisation of Brn-3b protein (brown stain) with VSMC marker, αSMA (magenta). Negative control = 2nd Ab and α-SMA. Different layers of the aorta: TI tunica intima, TM tunica media, TA tunica adventitia. c quantification of Brn-3b mRNA in primary VSMC cultures prepared using aortas from WT mutant n-3b KO mice (n = 5 independent cultures). Analyses were carried out using student’s t-test. d Representative immunofluorescent images of aortic VSMCs from WT mice (top panel) or Brn-3b KO mice, co-stained with Brn-3b (green) and α−SMA (red). DAPI mount indicates cell nuclei (blue).

Back to article page