Fig. 4: Analysis of RNA seq data using iDEP9.1 software. | Cell Death & Disease

Fig. 4: Analysis of RNA seq data using iDEP9.1 software.

From: Vascular dysfunction caused by loss of Brn-3b/POU4F2 transcription factor in aortic vascular smooth muscle cells is linked to deregulation of calcium signalling pathways

Fig. 4

a Principal Component Analysis (PCA) of all data obtained from RNA sequencing of WT and Brn-3b KO aortas. Samples from all three KO aortas (orange dots) were clustered together (boxed area) but only two WT samples (oval) showed similar patters while the WT sample 2 M (circled), was identified as an outlier with significant differences in both PC1 and PC3 components and was excluded from analyses. b Heat map showing the four clusters arising from K-means clustering of the top 2500 from two WT and three KO samples (excluding outlier sample, 2 M WT). KEGG pathways associated with enriched genes within each cluster are shown i.e. Red = upregulated genes; green = down regulated genes.

Back to article page