Fig. 6: Changes in serum let-7a and IL-6 levels are associated with radiosensitivity in esophageal squamous cancer. | Cell Death & Disease

Fig. 6: Changes in serum let-7a and IL-6 levels are associated with radiosensitivity in esophageal squamous cancer.

From: Mutual communication between radiosensitive and radioresistant esophageal cancer cells modulates their radiosensitivity

Fig. 6

A Pre- and post-radiotherapy serum let-7a levels were determined using real-time RT-PCR. B Pre- and post-radiotherapy serum IL-6 levels were quantified using ELISA. C The percentage decrease in serum let-7a levels was determined 7 days following initiation of radiotherapy and after the completion of radiotherapy. The percentage decrease in serum let-7a levels was calculated as follows: (pre-radiotherapy serum let-7a level − serum let-7a levels at 7 days after the initiation of radiotherapy or after the completion of radiotherapy)/pre-radiotherapy serum let-7a level × 100%. D The percentage increase in serum IL-6 levels was determined 7 days following initiation of radiotherapy and after the completion of radiotherapy. The percentage increase in serum IL-6 levels was calculated as follows: (serum IL-6 levels at day 7 following initiation of radiotherapy or after the completion of radiotherapy—pre-radiotherapy serum IL-6 level)/pre-radiotherapy serum IL-6 level × 100%. *P < 0.05, **P < 0.01, ns, not significant (two-sided Student’s t test). E The percentage decrease in serum let-7a levels induced by 60 Gy of radiotherapy was inversely correlated with tumor regression. The percentage decrease in serum let-7a levels induced by 60 Gy of radiotherapy was calculated as follows: (pre-radiotherapy serum let-7a level—post-radiotherapy serum let-7a level)/pre-radiotherapy serum let-7a level × 100%. The percentage decrease in the tumor diameter 2 months post radiotherapy was calculated as follows: (the baseline longest diameter of the tumor—the longest diameter of the tumor at 2 months post radiotherapy)/the baseline longest diameter of the tumor × 100%. F The percentage increase in serum IL-6 levels induced by 60 Gy of radiotherapy was inversely correlated with tumor regression. The percentage increase in serum IL-6 levels induced by 60 Gy of radiotherapy was calculated as follows: (post-radiotherapy serum IL-6 level − pre-radiotherapy serum IL-6 level)/pre-radiotherapy serum IL-6 level × 100%. G The percentage decrease in serum let-7a levels induced by 10 Gy of radiotherapy was inversely correlated with tumor regression. The percentage decrease in serum let-7a levels induced by 10 Gy of radiotherapy was calculated as follows: (pre-radiotherapy serum let-7a level – serum let-7a level after 10 Gy radiotherapy)/pre-radiotherapy serum let-7a × 100%. H The percentage increase in serum IL-6 levels induced by 10 Gy of radiotherapy was inversely correlated with tumor regression. The percentage increase in serum IL-6 levels induced by 10 Gy of radiotherapy was calculated as follows: (serum IL-6 level after 10 Gy of radiotherapy—re-radiotherapy serum IL-6 level)/pre-radiotherapy serum IL-6 level × 100%.

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