Fig. 6: Exogenous interaction relationship among TRIM38 (Flag tag), MITA (HA tag) and K48 plasmid (His tag) in 293 T cells before and after knockdown of three domains.

A Exogenous interaction relationship among TRIM38 (Flag tag), MITA (HA tag) and K48 plasmid (His tag). B Exogenous interaction relationship among knockdown of the ring finger structure of TRIM38 (Flag-TRIM38(d-RF)), MITA (HA-MITA) and K48 plasmid (His-K48). C Exogenous interaction relationship among knockdown of the B-box structure of TRIM38 (Flag-TRIM38(d-BB)), MITA (HA-MITA) and K48 plasmid (His-K48). D Exogenous interaction relationship among TRIM38 (Flag-TRIM38 (d-SP)), MITA (HA-MITA) and K48 plasmid (His-K48) with knockdown of the SPRY structure. E Expression validation of the type K48 ubiquitinated plasmid (His-K48) in 293 T cells (input).