Fig. 7: The relationship between pyroptosis and K48. | Cell Death & Disease

Fig. 7: The relationship between pyroptosis and K48.

From: Investigation into the role of the MITA-TRIM38 interaction in regulating pyroptosis and maintaining immune tolerance at the maternal-fetal interface

Fig. 7

A Morphology and number of pyroptotic cells in the supernatants of wild-type M1, M1 with DMSO (M1-vehicle), M1 with VX765 (M1-VX765) and wild-type M2 macrophages under a ×40 microscope. B Differences in the protein expression levels of GSDMD-N, caspase-1 and cleaved caspase-1 in the supernatants of M1, M1 with DMSO (M1-vehicle), M1 with VX765 (M1-VX765) and M2 macrophages. C Differences in the protein expression levels of MITA and cGAS in M1 macrophages, M1 macrophages treated with DMSO (M1-vehicle), M1 macrophages treated with VX765 (M1-VX765) and M2 macrophages. D Differential expression of MITA by ubiquitination of type K48 in M1, M1 with DMSO (M1-vehicle) and M1 with VX765 (M1-VX765) macrophages. E FCM analysis shows wild-type M1, M1 with DMSO (M1-vehicle), M1 with VX765 (M1-VX765) of FVS-labeled live cells, and the content of macrophages labeled by CD86 or CD209 in viable cells in the above three kinds of cells. a–c shows that the percentages of FVS (−) in M1, M1-vehicle and M1-VX765 are 59.5%, 58.9% and 58.2%, respectively. d–f shows that the percentages of macrophages showing the FVS (−) survival status represented by CD86 (+) macrophages in M1, M1-vehicle and M1-VX765 are 49.1%, 48.2% and 42.5%, respectively. g–i shows that the percentages of macrophages showing the FVS (−) survival status represented by CD209 (+) macrophages in M1, M1-vehicle and M1-VX765 are 0.09%, 0.10% and 0.86%, respectively. F The phenotypes of CD86 and CD209 in wild-type M1, M1-vehicle, and M1-VX765 cells were analyzed by FCM. *P < 0.05, ****P < 0.0001, ns = nonsignificant.

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