Fig. 2: MIF is located and forms ring-like structures at the proximal ends of the centrioles.

Representative images of RCTE cells stained with MIF antibody and co-stained with Ninein (a) or CEP164 (b) under 2D microscope. Scale bars, 5 μm. c Representative images of RCTE cells stained with MIF antibody and co-stained with CEP164 (red), which were visualized under 3D-SIM microscopy. Scale bars, 500 nm. The intensity plots of the rings in 3D images (c) from top (d)and side (e) views. f Representative images of RCTE cells stained with MIF antibody and co-stained with Ninein (red), which were visualized under 3D-SIM microscopy. Scale bars, 500 nm. The intensity plots of the rings in 3D images (f) from top (g) and side (h) views. i Representative images of RCTE cells stained with MIF antibody and co-stained with C-Nap1 (red), which were visualized under 3D-SIM microscopy. Scale bars, 500 nm. The intensity plots of the rings in 3D images (i) from top (j) and side (k) views. l Schematic of the localization of MIF, CEP164, Ninein, and C-Nap1 at centrosomes. The “top or side” views are regarding the centriole structures. DA distal appendage, SDA subdistal appendage.