Fig. 3: Screening of pyroptosis resistant cell lines induced by HGS-ETR1/2. | Cell Death & Disease

Fig. 3: Screening of pyroptosis resistant cell lines induced by HGS-ETR1/2.

From: Carboxypeptidase A4 negatively regulates HGS-ETR1/2-induced pyroptosis by forming a positive feedback loop with the AKT signalling pathway

Fig. 3

a After screening HLCZ01 cells for drug resistance, 2 μg/mL HGS-ETR1/2 was added to treat HLCZ01 and drug-resistant HLCZ01 cells (HLCZ01-ETR1/2 R) for 8 h, and the pyroptosis of the cells was observed by bright field photography and PI/Hoechst fluorescence photography. Scale bar, 125 μm. b, c HLCZ01 and HLCZ01-ETR1/2 R cells were treated with 2 μg/mL HGS-ETR1/2 for 8 h. GSDME cleavage was detected by WB, and β-actin was used as the internal reference protein. d The mRNA expression levels of DR4, DR5 and GSDME in HLCZ01 and HLCZ01-ETR1/2 R cells were detected by qPCR, with GAPDH as the internal reference gene. e The protein expression levels of DR4, DR5 and GSDME in HLCZ01 and HLCZ01-ETR1/2 R cells were detected by western blotting, and β-actin was used as the internal reference protein. Three independent biological replicates were performed for each of the above experiments. Two-sided Student’s t test was used to analyse significant differences (b-e) (*p <0.05, **p <0.01).

Back to article page