Fig. 6: Increased FTO expression destabilizes PTEN mRNA in neonatal rats after HI. | Cell Death & Disease

Fig. 6: Increased FTO expression destabilizes PTEN mRNA in neonatal rats after HI.

From: N6-methyladenosine demethylase FTO regulates synaptic and cognitive impairment by destabilizing PTEN mRNA in hypoxic-ischemic neonatal rats

Fig. 6

A, B Predicted m6A site in PTEN mRNA of a sequence-based m6A modification site predictor (SRAMP). C After OGD-R treatment, primary hippocampal neurons were harvested for real-time quantitative polymerase chain reaction (RT-qPCR) to assess PTEN expression, and loading control GAPDH. D, E PTEN levels in the ipsilateral hippocampus were measured by western blot. F PTEN mRNA half-life after FTO overexpression measured by incubating cells with actinomycin D, extracting total RNA at the times shown, and measuring PTEN and (housekeeping) GAPDH mRNA levels by RT-qPCR analysis. G The relative levels of m6A in PTEN mRNA were tested by MeRIP-qPCR from primary cultured hippocampal neurons with overexpression of FTO. *p < 0.05 vs. the Sham group; #p < 0.05 vs. the HI+Lv.GFP group.

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