Fig. 1: Impact of Gss deficiency in germ cells on fertility. | Cell Death & Disease

Fig. 1: Impact of Gss deficiency in germ cells on fertility.

From: Gss deficiency causes age-related fertility impairment via ROS-triggered ferroptosis in the testes of mice

Fig. 1

A Schematic diagram of generating a Gss-Flox mouse model by CRISPR/Cas9 system. B Agarose gel analysis of PCR products amplified used primer F1, R1, and Cre F, R. Wild-type allele: one band with 138 bp; Heterozygotes: two bands with 200 bp and 138 bp; Homozygotes: one band with 200 bp. C Testicular sections of S8/Control and S8/Gss−/− mice for Gss (red), γH2ax (green), and DAPI (blue) staining. The two columns on the right are enlarged images in a white dotted box. Scar bar = 50 μm. D The statistic for fluorescence intensity of GSS in various germ cells. ***P < 0.001; more than 40 cells were counted from 4 biologically independent animals for every group. E Litter size of 2- and 8-month-old S8/Control and S8/Gss−/− male mice that were caged with WT females for 3 months. ***P < 0.001; NS indicates non-significant, n = 4 (biologically independent animals). F The testes of S8/Gss−/− mice were smaller than those of S8/Control mice at 8 months of age. G The testicular weight of the S8/Control and S8/Gss−/− mice, at 2, 4, and 8 months. **P < 0.01; NS indicates non-significant; n = 8 (testes from eight different animals).

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