Fig. 3: Gss deficiency affects meiosis during spermatogenesis. | Cell Death & Disease

Fig. 3: Gss deficiency affects meiosis during spermatogenesis.

From: Gss deficiency causes age-related fertility impairment via ROS-triggered ferroptosis in the testes of mice

Fig. 3

A–C Immunofluorescence staining of PCNA in testicular sections from 2- and 8-month-old mice. Scar bar = 50 μm. ***P < 0.001; NS indicates non-significant, n = 3 (biologically independent animals); more than ten tubules were counted for every mouse. D–F The immunohistochemical assay for c-KIT was used to examine spermatogonia differentiation. Scar bar = 50 μm. NS indicates non-significant, n = 3 (biologically independent animals); more than ten tubules were counted for every mouse. G–I Immunofluorescence detection of γH2AX and the statistical results in testis from S8/Control and S8/Gss−/− mice at 2 or 8 months. J–L The test and count results of spermatocytes with punctate γH2AX expression in the testes of 2- and 8-month-old mice. G–L Scar bar = 50 μm. ***P < 0.001; NS indicates non-significant, n = 4 (biologically independent animals); more than ten tubules were counted for every mouse. M Diagram of the relative number of germ cells in the testis of 8-month-old S8/Control and S8/Gss−/− mice.

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