Fig. 4: Analysis of sperms from 2- and 8-month-old mice. | Cell Death & Disease

Fig. 4: Analysis of sperms from 2- and 8-month-old mice.

From: Gss deficiency causes age-related fertility impairment via ROS-triggered ferroptosis in the testes of mice

Fig. 4

A Sperm concentration in cauda epididymis of S8/Control and S8/Gss−/− mice at 2 and 8 months of age under the same treatment condition. NS indicates non-significant, **P < 0.01; n = 6 (biologically independent animals). B, C CASA analysis of sperms from cauda epididymis of 2- and 8-month-old mice. NS indicates non-significant, n = 6 (biologically independent animals). D, E The H&E staining of sperms from cauda epididymis of S8/Control and S8/Gss−/− mice at 2 or 8 months. Scar bar = 20 μm. F The proportion of malformed sperms in 8-month-old mice, and G the proportion of different types of malformed sperms. **P < 0.01, ***P < 0.001; NS indicates non-significant, n = 6 (biologically independent animals). H SEM images of sperms from 8-month-old S8/Control and S8/Gss−/− mice. Scar bar = 5 μm. PAS post acrosomal segment, EQ equatorial segment, AA anterior acrosome, HR hook rim, VS ventral spur. I The PNA staining of sperms of 8-month-old S8/Control and S8/Gss−/− mice, and J the proportion of abnormal morphological acrosomes. The images on the bottom are the enlarged view in the white dotted boxes. Scar bar = 10 μm. ***P < 0.001; n = 4 (biologically independent animals); more than 200 sperms were counted for each mouse. K The PNA staining of testicular slices was used to examine acrosome development in mice. The red arrow points to the abnormally located acrosome. Scar bar = 10 μm.

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