Fig. 7: The ferroptosis levels were examined following GSH and Fer-1 treatment. | Cell Death & Disease

Fig. 7: The ferroptosis levels were examined following GSH and Fer-1 treatment.

From: Gss deficiency causes age-related fertility impairment via ROS-triggered ferroptosis in the testes of mice

Fig. 7

A–C Western blot was used to detect the protein levels of GPX4 and ALOX15. *P < 0.05, **P < 0.01, ***P < 0.001; n = 4 (biologically independent animals). D–F The lipid peroxidation markers 3-NT and 4-HNE in mice testis were determined by western blotting. **P < 0.01, ***P < 0.001, n = 4 (biologically independent animals). G The relative levels of ROS in testis of S8/Control, S8/Gss−/− mice, S8/Gss−/− mice + GSH, and S8/Gss−/− mice + Fer-1 mice. H The contents of 8-OHdG of testis were examined by assay kit and normalized by S8/Control group. I The levels of Fe2+ were examined in the testis of mice treated with GSH and Fer-1. G–I *P < 0.05, **P < 0.01, ***P < 0.001; n = 4 (biologically independent animals).

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