Fig. 4: Mutations within the E285 cluster show high prevalence in melanoma and other cancer types.

A The cluster of amino acid mutations within p53 that can have a similar functional effect on the C-terminal helix of the DBD domain as E285K. The E285K mutation is shown in light red, all other mutations in dark red. Mutant residues forming contacts interacting with DNA in the DNA-bound p53 complex are indicated (“DNA interface”). B Prevalence of E285-cluster mutations and in particular E258K mutation compared to other p53 mutation variants in MM, and C the frequency of most common p53 mutation in MM was correlated with the patient outcome (alive vs death) https://www.cbioportal.org/study/summary?id=msk_met_2021. Filled red arrows point at mutations being present in the E285 cluster, open red arrows at designated gain-of-function mutations. D The frequency of p53 missense mutations in various cancer tissues. The ratio of E285* (light red), of mutations within the E285 cluster (dark red), and all other p53 mutations (gray) as derived from https://tp53.isb-cgc.org is shown.