Fig. 4: POM121 regulates nuclear transport of PPARγ.
From: Nuclear pore protein POM121 regulates subcellular localization and transcriptional activity of PPARγ
A POM121 KO promotes nuclear translocation of PPARγ. Clonal cells were subjected to IF microscopy using PPARγ and Abs directed against the NT or CT of POM121. Data are mean numbers of PPARγ+ signals per cell (= DAPI+ nucleus) ± S.E. (n ≥ 5 cells per field, n ≥ 15 fields; *p < 0.05 vs. EV, Fisher Exact test and 2way-ANOVA with Bonferroni post-tests, n = 2 per clone). B Representative images. Color code: yellow = POM121/PPARγ (overlay); green = PPARγ; red = POM121; blue = nuclei (DAPI); scale bar = 50 µm; original magnifications 630x (with zoom-in). Arrows mark cytoplasmic (abbrev. “cyt”) PPARγ in EV vs. nuclear (abbrev. “nuc”) PPARγ in KO cells and perinuclear POM121+ punctae.
