Fig. 6: Structure prediction models of POM121 holo protein.

A I-TASSER query of FL POM121 protein [UniProt ID: Q96HA1 (P121A_HUMAN)]. Top 5 theoretical 3D models are listed (Table S13) with C-scores [-5 (low) to +2 (high)] for model confidence: Model (Rank A) -1.19 [3gavA] is shown. Putative sequences of TM (red), NLS (green), PPARγ-binding (blue) and FG-rich (orange) domains of POM121 were mapped to model Rank A. Note the ordered N-terminal (NT) domains vs. the disordered/unspecified (low confidence) structure of the C-terminus (CT, black brackets). B B-Factor graph of FL POM121 protein [UniProt ID: Q96HA1 (P121A_HUMAN)] in the top theoretical model (Rank A) with confidence [y-axis: - (ordered) to + (disordered)]. B-Factor was lowest (most reliable) along the N-terminal TM domain, whereas peaked (most disordered) toward the C-terminus (aa 1225–1249), corresponding to the elongated irregular shape of the model in (A), consistent with the FG-repeats of the hydrophilic “basket” of the NPC protein. C Local Quality Estimate plot of FL POM121C protein [UniProt ID: A8CG34 (P121C_HUMAN)] for the theoretical model AF-A8CG34-F3 given by SWISS-MODEL (alpha-fold DB: average model confidence = 43.19). Legend: TM = G27 to W67 (helical); NLS (partial) = E287 to D306 (helical), PPARγ-binding peptides = M371 to S477 (disordered). D 3D model of FL POM121C protein [UniProt ID: A8CG34 (P121C_HUMAN)] given by SWISS-MODEL (alpha-fold DB in C). Model Confidence: Light blue = very high (pLDDT > 90); Dark blue = confident (90 > pLDDT > 70); Gray = low (70 > pLDDT > 50); Orange = very low (pLDDT < 50). N-terminal TM and NLS helices are highlighted as shaded blue rectangles, the N-terminus orientated towards the ER cisterna side in yellow. Note that the sequence consecutive of the NLS was predicted to be mostly disordered, including the PPARγ-peptide binding regions identified by MS (Table S3) (S1). The third high-confidence helix did not overlap with the putative PPARγ-binding peptides.