Fig. 2: NEDD8−/− mice showed diminished postoperative pulmonary metastasis of colon cancer cells. | Cell Death & Disease

Fig. 2: NEDD8−/− mice showed diminished postoperative pulmonary metastasis of colon cancer cells.

From: Targeting NEDD8 suppresses surgical stress-facilitated metastasis of colon cancer via restraining regulatory T cells

Fig. 2

NEDD8+/+ and NEDD8−/− mice were randomly subjected to sham or laparotomy surgery after the inoculation of CFSE-labeled MC38 cells (n = 6 per group). A Representative images of the metastatic foci on murine lung surfaces on D3 and the statistical data of all mice in each group. B Representative images of the H&E staining of the murine lungs on D3 and the calculation of the percentage of metastatic area relative to total lung section (n = 6 per group). C The number of CFSE-labeled MC38 cells in murine lungs were detected by flow cytometry showing the MFI of CFSE 6 h, 12 h, and 24 h post MC38 injection (n = 6 per group). D CFSE-labeled MC38 cells in murine lungs 6 h, 12 h, and 24 h post MC38 injection were detected by immunofluorescence (n = 6 per group). Green: CFSE, blue: DAPI. Representative images were shown. All experiments were repeated for three times. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Back to article page