Fig. 2: DR5 gene deletion reduces the number of intestinal epithelial cells via targeting ISC. | Cell Death & Disease

Fig. 2: DR5 gene deletion reduces the number of intestinal epithelial cells via targeting ISC.

From: Death receptor 5 is required for intestinal stem cell activity during intestinal epithelial renewal at homoeostasis

Fig. 2

Data were expressed as mean ± SEM. Statistical analyses were performed by the unpaired t-tests (*P < 0.05, ***P < 0.001, ****P < 0.0001). A Representative images of H&E staining in the ileum of WT mice and DR5-/- mice (scale bars: 100 μm). B Quantification of villus length within two groups (n = 6). At least 15 correctly aligned villi were counted randomly for each slide and the mean value was calculated. C The total number of epithelial cells in ileal villus of WT mice and DR5-/- mice (n = 6). At least 15 correctly aligned villi were measured for every slide to get the mean value. D qRT-PCR (n = 6) and western blot (n = 4) were performed to determine the relative mRNA and protein levels of lysozyme (Lyz) in ileum of WT mice and DR5-/- mice. Lyz is the specific marker for Paneth cells. E qRT-PCR was performed to determine the relative mRNA levels of Muc2 and Tff3 in ileum of WT mice and DR5-/- mice (n = 6). Both are the specific marker genes for goblet cells. F Western blot to quantify the protein expression of MUC2 in ileum of WT mice and DR5-/- mice (n = 4). G qRT-PCR (n = 6) and western blot (n = 4) were performed to determine the relative mRNA and protein expression of ChgA in ileum of WT mice and DR5-/- mice. It is the specific marker for enteroendocrine cells. H qRT-PCR was performed to determine the relative mRNA levels of Alpi in ileum of WT mice and DR5-/- mice (n = 6). It is the specific marker for enterocytes. I Relative mRNA expression of inflammatory factors in the ileum of WT mice and DR5-/- mice (n = 6). J Quantification of TUNEL+ cells in ileal villus of WT mice and DR5-/- mice (n = 6). At least 10 villi were counted randomly for each slide and the mean value was calculated. K Total number of epithelial cells in ileal crypts of WT mice and DR5-/- mice (n = 6). At least 15 correctly aligned crypts were measured for every slide to get the mean value. L The volcano plot visualized the DEGs in ileal crypts from WT mice and DR5-/- mice. Names listed in the figure were DEGs associated with proliferation or differentiation of ISC.

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