Fig. 7: DR5 is involved in the regulation of Wnt, Notch and BMP pathway in vivo and in ex vivo organoids. | Cell Death & Disease

Fig. 7: DR5 is involved in the regulation of Wnt, Notch and BMP pathway in vivo and in ex vivo organoids.

From: Death receptor 5 is required for intestinal stem cell activity during intestinal epithelial renewal at homoeostasis

Fig. 7

Data were expressed as mean ± SEM. Statistical analyses were performed by the unpaired t-tests (*P < 0.05, **P < 0.01, ***P < 0.001). A qRT-PCR analysis of β-catenin, Tcf4 and c-Myc in ileal crypts of WT mice and DR5-/- mice (n = 6). They are Wnt signalling target genes. B qRT-PCR analysis of Hes1 and Hes5 in ileal crypts of WT mice and DR5-/- mice (n = 6). They are Notch signalling target genes. C qRT-PCR analysis of Msx1 in ileal crypts of WT mice and DR5-/- mice (n = 6). It is the BMP signalling target gene. D Effect of Bioymifi (100 nM) administration in vitro on the mRNA expression of β-catenin, c-Myc, Hes1 and Msx1 in organoids (n = 6). E Effect of Bioymifi (100 nM) administration in vitro on the protein expression of β-catenin and c-Myc in organoids (n = 4). F Effect of Bioymifi (100 nM) administration in vitro on the protein expression of Hes1 in organoids (n = 4). G Effect of Bioymifi (100 nM) administration in vitro on the protein expression of p-Smad1/5 in organoids (n = 4).

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