Fig. 1: The protein level of TRIM65 was suppressed during II/R and H/R. | Cell Death & Disease

Fig. 1: The protein level of TRIM65 was suppressed during II/R and H/R.

From: E3 ligase TRIM65 alleviates intestinal ischemia/reperfusion injury through inhibition of TOX4-mediated apoptosis

Fig. 1

A Expression of TRIM65 protein in Caco-2 cells subjected to different times (0, 1, 2, 4, 6, 12, or 24 h) of reoxygenation following 24 h of hypoxia was detected by western blot. B TRIM65 protein expression in IEC-6 cells subjected to different times of reoxygenation following 24 h of hypoxia as indicated was detected by western blot. C The expression of TRIM65 protein in intestine tissue after 60 min of ischemia followed by 1, 6, 12 and 24 h of reperfusion was detected by western blot. D H&E staining of the intestine and histologic injury scores of the intestine in sham and II/R groups were quantified as described in Materials and Methods. Scale bar, 50 μm. E TRIM65 protein localization and expression in the intestine were detected by immunohistochemistry. Scale bar, 50 μm. All results are expressed as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 by Student’s t test.

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