Fig. 4: TRIM65 mediates H/R-induced apoptosis of intestinal epithelial cells. | Cell Death & Disease

Fig. 4: TRIM65 mediates H/R-induced apoptosis of intestinal epithelial cells.

From: E3 ligase TRIM65 alleviates intestinal ischemia/reperfusion injury through inhibition of TOX4-mediated apoptosis

Fig. 4

A Apoptosis of intestinal epithelial cells IEC-6 overexpressing TRIM65 or control cells caused by H/R were measured by flow cytometry using annexin V/PI staining. B The apoptotic cells (annexin V+PI+ and annexin V+PI- cells) were analyzed. The numbers in each plot indicate the percentage of positive cells. C Expressions of TRIM65, BAX, and Bcl-2 protein in TRIM65 overexpressing intestinal epithelial cells after H/R were detected by western blot. D Quantification of the western blot bands was carried out using Gel-Pro Analyzer software, and results were presented as “fold changes” on the right side of the bands. E, F The extent of apoptosis in intestinal epithelial cells with lowered TRIM65 expression after H/R was analyzed by flow cytometry. G, H Expressions of TRIM65, BAX, and Bcl-2 protein in intestinal epithelial cells with TRIM65 knockdown after H/R were detected by western blot. Quantification of the western blot bands was carried out using Gel-Pro Analyzer software, and results were presented as “fold changes” on the right side of the bands. All results are expressed as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA followed by Tukey’s test.

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