Fig. 5: TRIM65 directly interacts with TOX4. | Cell Death & Disease

Fig. 5: TRIM65 directly interacts with TOX4.

From: E3 ligase TRIM65 alleviates intestinal ischemia/reperfusion injury through inhibition of TOX4-mediated apoptosis

Fig. 5

A The interaction between TRIM65 and TOX4 was detected by yeast two-hybrid assay. Proteins 53 and T or Lam were selected as positive and negative controls, respectively. B, C HEK293T cells transfected with HA-TOX4 or Flag-TRIM65 and treated with MG132, then a co-IP experiment was performed to examine the interaction of TRIM65 with TOX4. D Confocal microscopy detected the localization of TOX4 and TRIM65 in intestinal epithelial cells. E The interaction of TRIM65 with TOX4 in intestinal epithelial cells with or without H/R was detected. F GST pull-down assay revealed the physical interaction between TRIM65 and TOX4. G Detection of the physical interaction of deletion mutants of TOX4 with TRIM65 by GST pull-down assay. H Schematic of interaction between TRIM65 and the serial deletion mutants of TOX4 fused with GFP. I HEK293T cells were transfected with TOX4 and GFP-TRIM65 deletion mutants and treated with MG132. The interaction between TOX4 and different TRIM65 deletion mutants was detected by co-IP. J Schematic of the interaction between TOX4 and the serial deletion mutants of TRIM65.

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