Fig. 7: TRIM65 targets TOX4 to promote II/R-induced apoptosis in intestinal epithelial cells. | Cell Death & Disease

Fig. 7: TRIM65 targets TOX4 to promote II/R-induced apoptosis in intestinal epithelial cells.

From: E3 ligase TRIM65 alleviates intestinal ischemia/reperfusion injury through inhibition of TOX4-mediated apoptosis

Fig. 7

A Expressions of BAX, Bcl-2, Flag and GFP proteins in intestinal epithelial cells transfected with GFP-TOX4 or Flag-TRIM65 were detected by western blot. B Quantification of the bands was carried out using Gel-Pro Analyzer software. C Flow cytometry analysis of the extent of apoptosis in TRIM65 or TOX4 overexpressing intestinal epithelial cells caused by H/R. D The apoptotic cells (annexin V+PI+ and annexin V+PI- cells) were analyzed. The numbers in each plot indicate the percentage of positive cells. E Expressions of BAX, Bcl-2, TRIM65 and TOX4 protein in intestinal epithelial cells with lowered TOX4 expression were detected by western blot. F Quantification of the bands was carried out using Gel-Pro Analyzer software. G Flow cytometry observation of the extent of apoptosis in intestinal epithelial cells with downregulated TRIM65 or TOX4 expression after H/R. H The apoptotic cells (annexin V+PI+ and annexin V+PI- cells) were analyzed. I TRIM65 and TOX4 protein expressions in the intestine after II/R were detected by IHC. J Correlation analysis between the protein levels of TRIM65 and TOX4 in II/R intestine tissue. All results are expressed as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA followed by Tukey’s test.

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