Fig. 2: Palmitate induces a caspase-dependent trophoblast lipoapoptosis.
From: Palmitate induces integrated stress response and lipoapoptosis in trophoblasts
A JEG-3, C JAR cells and D HTR-8 were treated with either vehicle (Veh), palmitate (PA), Z-VAD-fmk, or PA + Z-VAD for 24 h. Percent apoptotic nuclei (left panel) and caspase 3/7 activity (right panel) were significantly elevated with PA-treated cells. Cotreatment of PA and Z-VAD-fmk prevents PA-induced trophoblast lipoapoptosis. B. JEG-3 cells were treated with either Veh, PA (200–800 µM), Z-VAD-fmk or PA (400 µM) + Z-VAD-fmk for 24 h and viability was analyzed using CellTiter-Glo 2.0. PA treatment significantly reduced intracellular ATP levels, while co-treatment of Z-VAD-fmk with PA (400 µM) restored intracellular ATP levels, compared with vehicle-treated cells (Veh). Data presented as mean ± SEM, n = 4. *P < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 compared to vehicle-treated cells or PA-treated cells.
