Fig. 7: Palmitate activates stress granule formation in trophoblast.
From: Palmitate induces integrated stress response and lipoapoptosis in trophoblasts
A JEG-3 cells were treated with 400 μM of palmitate (PA) for a period of 24 and the cells were stained with G3BP1 and TIA1 showed increased punctae in PA-treated cells compare to vehicle-treated cells. DAPI was used for nuclear staining. Merge of G3BP1 and TIA1 showed increased colocalization of both staining (purple color). The images are representative images. B, C Cells were treated with 400 μM of palmitate (PA) and with the cotreatment of PA and stress granule inhibitor (GSK260) and stress granule activator (PGJ2) for a period of 24 h and apoptosis was quantitated using nuclear morphological changes (B) and caspase 3/7 activity (C). Palmitate-induced trophoblast lipoapoptosis is blocked with the activation of stress granule formation using PGJ2. Data presented as mean ± SEM, n = 6. *P < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 compared to vehicle-treated cells or PA-treated cells.
