Fig. 1: CYLD interacts and inhibits the activity of class I HDACs. | Cell Death & Disease

Fig. 1: CYLD interacts and inhibits the activity of class I HDACs.

From: CYLD induces high oxidative stress and DNA damage through class I HDACs to promote radiosensitivity in nasopharyngeal carcinoma

Fig. 1

a A volcano of differentially expressed genes identified by mRNA-seq in control and CYLD overexpressing NPC cells. b GSEA analysis of CYLD relative pathways. c HDACs activity measurement in control and CYLD knockdown cells. d HDACs activity measurement in control, CYLD, and CYLD-C601A overexpressing cells. e Proximity ligation assay indicating the interaction of CYLD and HDAC1/2 in HK1 cells (red: PLA positive signal; blue: DAPI, scale bar = 10 μm). f HONE1 cells were disrupted. The cell lysates were subjected to immunoprecipitation (IP) with anti-HDAC1/2. g HONE1-EBV cells transfected with the indicated plasmids, acetyl-Lys (Ac-K) was immunoprecipitated and probed with HDAC1 antibodies. The whole cell extract was used as input. h Schematic representation of the Flag-tagged full length CYLD (FL) and its various deletion mutants, including N-terminal 1-303 deletion (△N), middle domain deletion (△M), and C-terminal deletion(△C) constructs. i 293 T cells transfected with the indicated constructs were disrupted. The cell lysates were subjected to immunoprecipitation with anti-Flag.

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